Journal of Clinical Laser Medicine & Surgery Volume 20, No 2: 83–87 (2002)
Aurelí Cio N. Silva Junior M.S., Antonio L.B. Pinheiro, Ph.D., Marília G. Oliveira Ph.D., Ruben Weismann Ph.D., Luciana Maria Pedreira Ramalho, Ph.D., and Renata Amadei Nicolau, M.S.
Objective: The aim of this study was to evaluate morphometricly the amount of newly formed bone after GaAlAs laser irradiation of surgical wounds created in the femur of rats. Background
Data: Low-level laser therapy (LLLT) has been used in several medical specialties because of its biomodulatory effects on different biological tissues. However, LLLT is still controversial because of contradictory reports. This is a direct result of the different methodologies used in these works.
Materials and Methods: In this study, 40 Wistar rats were divided into four groups of 10 animals each: group A (12 sessions, 4.8 J/ cm 2per session, observation time of 28 days); group C (three sessions, 4.8 J/ cm 2per session, observation time of 7 days). Groups B and D acted as non-irradiated controls. The specimens were routinely processed to wax and cut at 6-mm thickness and stained with H&E. For computerized morphometry, Imagelab ®software was used.
Results: Computerized morphometry showed a significant difference between the areas of mineralized bone in groups C and D ( p = 0.017). There was no difference between groups A and B (28 days; p = 0.383).
Conclusion: It is concluded that, under this experimental condition, LLLT increased bone repair at early bone healing.